| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; Hbubr1; Mitotic checkpoint kinase MAD3L; Protein SSK1; BUB1B; BUBR1, MAD3L, SSK1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects mitotic checkpoint serine/threonine-protein kinase BUB1 beta (gene BUB1B) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 119.5 kDa, and the supplier reports an observed band at about 120–130 kDa.
BubR1 (BUB1B) is a kinase-family protein of the spindle assembly checkpoint. As part of the mitotic checkpoint complex with MAD2, BUB3 and CDC20, it holds the anaphase-promoting complex (APC/C) inactive until every chromosome is correctly attached to the spindle, and it also helps recruit the motor CENPE to kinetochores. It is studied in mitosis, chromosome instability, aneuploidy and aging.
| Target | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (gene BUB1B; UniProt O60566, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 119.5 kDa |
| Observed MW | 120–130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human Hek293 cells, human K562 cells, human HL-60 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse testis tissue, rat testis tissue, human colon cancer tissue, human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human BubR1/BUB1B fragment (Lys26–Glu448), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitotic checkpoint serine/threonine-protein kinase BUB1 beta in the cytoplasm, nucleus and chromosome. Tissue expression noted by UniProt (human): Highly expressed in thymus followed by spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhenzhen Hao et al. (2025) A Comprehensive Analysis Revealing BUB1B as a Potential Prognostic and Immunological Biomarker in Lung Adenocarcinoma. International Journal of Molecular Sciences. 10.3390/ijms26052061
Yu Hou et al. (2025) Vitamin K2 mitigates cognitive, and motor impairments induced by multiple surgery with anesthesia and analgesia in neonatal stage. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2025.151784
