| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; Hbubr1; Mitotic checkpoint kinase MAD3L; Protein SSK1; BUB1B; BUBR1; MAD3L; SSK1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human BubR1/BUB1B recombinant protein (Position: K26-E448). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) is an antibody for BUB1B detection raised in Mouse (Monoclonal, clone Clone: 5I7, Mouse IgG1), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: BUB1B (BUB1 mitotic checkpoint serine/threonine kinase B); UniProt: O60566
- Antibody format: Mouse, Monoclonal, clone Clone: 5I7, Mouse IgG1
- Molecular weight: 130 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-BubR1/BUB1B Antibody Picoband® (monoclonal, 5I7) catalog # M01564-2.
Biological background
Biological context: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Expression and localization notes: cellular localization: Centrosome. Nucleus. Cytoplasm. Kinetochore., tissue context: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index..
Common research applications
- Western blotting (WB): Compare BUB1B levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of BUB1B in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify BUB1B-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Mitotic checkpoint serine/threonine-protein kinase BUB1 betais anenzymethat in humans is encoded by theBUB1Bgene. This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Centrosome. Nucleus. Cytoplasm. Kinetochore.
- Tissue details: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
- Research category: DNA/RNA,Epigenetics and Nuclear Signaling,RNA Processing
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
