| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta;2.7.11.1;MAD3/BUB1-related protein kinase;hBUBR1;Mitotic checkpoint kinase MAD3L;Protein SSK1;BUB1B;BUBR1, MAD3L, SSK1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human BubR1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABFG-2 is a rabbit monoclonal antibody that recognizes mitotic checkpoint serine/threonine-protein kinase BUB1 beta (gene BUB1B) in human, mouse and rat samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 119.5 kDa, and the supplier reports an observed band at about 120 kDa.
BubR1 (BUB1B) is a kinase-family protein of the spindle assembly checkpoint. As part of the mitotic checkpoint complex with MAD2, BUB3 and CDC20, it holds the anaphase-promoting complex (APC/C) inactive until every chromosome is correctly attached to the spindle, and it also helps recruit the motor CENPE to kinetochores. It is studied in mitosis, chromosome instability, aneuploidy and aging.
| Target | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (gene BUB1B; UniProt O60566, human) |
|---|---|
| Clone | ABFG-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 119.5 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human bubR1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mitotic checkpoint serine/threonine-protein kinase BUB1 beta in the cytoplasm, nucleus and chromosome. Tissue expression noted by UniProt (human): Highly expressed in thymus followed by spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Peng Tan et al. (2026) Discovery of furan-trimethoxyphenyl hybrids as novel colchicine-binding site inhibitors that induce mitotic catastrophe with potent anti-acute myeloid leukemia effect. Bioorganic Chemistry. 10.1016/j.bioorg.2026.110109
