| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7;FOS;G0S7; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human c-Fos |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IIO-6 is a rabbit monoclonal antibody that recognizes protein c-Fos in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 40.7 kDa, and the supplier reports an observed band at about 41 kDa.
c-Fos is a transcription factor that pairs with JUN family proteins to form the AP-1 complex, which binds DNA and regulates genes controlling proliferation, differentiation and survival. FOS is a classic immediate early gene rapidly induced by growth factors, stress and neuronal activity, so c-Fos expression is widely used as a marker of activated neurons.
| Target | Protein c-Fos (gene FOS; UniProt P01100, human) |
|---|---|
| Clone | IIO-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.7 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human c-Fos.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein c-Fos in the nucleus, endoplasmic reticulum and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Ming et al. (2026) Injectable hydrogel bioelectrostimulator for wireless deep brain neuromodulation. Nature Communications. 10.1038/s41467-026-69226-1
Wen-tao Zhang et al. (2024) Molecular characterization of PANoptosis-related genes in chronic kidney disease. PLoS One. 10.1371/journal.pone.0312696
Yi Ping Zheng et al. (2018) Tumor suppressive role of miR-569 in lung cancer. Oncology Letters. 10.3892/ol.2018.7869
