| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor AP-1;Activator protein 1;AP1;Proto-oncogene c-Jun;V-jun avian sarcoma virus 17 oncogene homolog;p39;JUN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human c-Jun |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CAO-10 is a rabbit monoclonal antibody that recognizes transcription factor Jun in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 35.7 kDa, and the supplier reports an observed band at about 43 kDa.
c-Jun (JUN) is a bZIP transcription factor and core component of AP-1. It forms dimers with Fos family proteins and other partners to bind TRE (TGA[GC]TCA) sites and control genes involved in proliferation, differentiation, stress responses and apoptosis. Its activity is strongly increased by JNK phosphorylation, and it is a widely used readout in studies of cell signaling and cancer.
| Target | Transcription factor Jun (gene JUN; UniProt P05412, human) |
|---|---|
| Clone | CAO-10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.7 kDa |
| Observed MW | 43 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human A431 cells, human U87 cells, human U2OS cells, human 293T- WT cells, human 293T-JUN KO cells, rat C6 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat cerebral cortex tissue, rat cerebellum tissue, human squamous carcinoma tissue, human breast cancer tissue, mouse skeletal muscle - gastrocnemius tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human c-Jun.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor Jun in the nucleus. Tissue expression noted by UniProt (human): Expressed in the developing and adult prostate and prostate cancer cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Hua Liu et al. (2024) Using broadly targeted plant metabolomics technology combined with network pharmacology to explore the mechanism of action of the Yishen Gushu formula in the treatment of postmenopausal osteoporosis in vivo. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118469
Shuang Hu et al. (2024) Study on therapeutic mechanism of total salvianolic acids against myocardial ischemia-reperfusion injury based on network pharmacology, molecular docking, and experimental study. Journal of Ethnopharmacology. 10.1016/j.jep.2024.117902
Yeke Wu et al. (2024) A breakthrough in periodontitis treatment: Revealing the pharmacodynamic substances and mechanisms of Kouqiangjie formula. Journal of Ethnopharmacology. 10.1016/j.jep.2024.117738
Jiejie Cui et al. (2020) All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members. Genes & Diseases. 10.1016/j.gendis.2019.12.012
Zhou Can-Can et al. (2022) Zinc supplementation ameliorates sorafenib-induced cognitive impairment through ROS/JNK signaling pathway. Biological Trace Element Research. 10.1007/s12011-022-03142-5
Binay Sevgi et al. (2022) Transcription factor Runx2 changes the expression of some matricellular proteins in metastatic breast cancer cells. Molecular Biology Reports. 10.1007/s11033-022-07457-3
Cao DW et al. (2019) Ambroxol alleviates ventilator-induced lung injury by inhibiting c-Jun expression. European Review for Medical and Pharmacological Sciences. 10.26355/eurrev_201906_18092
Li Ying-qin et al. (2018) Pre-Injection of Small Interfering RNA (siRNA) Promotes c-Jun Gene Silencing and Decreases the Survival Rate of Axotomy-Injured Spinal Motoneurons in Adult Mice. Journal of Molecular Neuroscience. 10.1007/s12031-018-1098-y
Chi Ma et al. (2014) Vasopressin decreases neuronal apoptosis during cardiopulmonary resuscitation. Neural Regeneration Research. 10.4103/1673-5374.130107
