Anti-c-Jun Rabbit Monoclonal Antibody

Overview

Anti-c-Jun Rabbit Monoclonal Antibody is an antibody targeting JUN. Common applications include WB, IHC, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: CAO-10; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 43 kDa; calculated MW: 35676 MW.

Boster Bio Anti-c-Jun Rabbit Monoclonal Antibody catalog # M02038. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: JUN — Transcription factor AP-1
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: CAO-10; Isotype: Rabbit IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 43 kDa; Calculated: 35676 MW

Biological background

Protein function (datasheet): Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). .

Cellular localization (datasheet): Nucleus.

Tissue details (datasheet): Expression is restricted to melanoma and melanocyte cell lines and retina.

Research relevance and current trends

• Commonly studied in contexts related to Hlh/Leucine Zipper,Immunology,Innate Immunity,Nuclear Signaling Pathways,Oncoproteins,Oncoproteins/Suppressors,Signal Transduction,Signaling Pathway,TLR Signaling,Transcription,Transcription Factors.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.