| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor Maf;Proto-oncogene c-Maf;V-maf musculoaponeurotic fibrosarcoma oncogene homolog;MAF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human c-Maf |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DHI-13 is a rabbit monoclonal antibody that recognizes transcription factor Maf in human, mouse and rat samples and is validated for IHC. The predicted molecular weight is 38.5 kDa, and the supplier reports an observed band at about 38 kDa.
MAF (c-Maf) is a large Maf-family basic leucine zipper transcription factor. It regulates crystallin genes during lens fiber development and controls key immune genes, notably IL-4 in T helper 2 cells and IL-10 in several immune cell types, and it is also studied in multiple myeloma, where MAF translocations occur.
| Target | Transcription factor Maf (gene MAF; UniProt O75444, human) |
|---|---|
| Clone | DHI-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 38.5 kDa |
| Observed MW | 38 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Immunohistochemistry | 1:50–1:200 |
|---|
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human c-Maf.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor Maf in the nucleus. Tissue expression noted by UniProt (human): Expressed in endothelial cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mitsunori Fujino et al. (2023) Transcription factor c-Maf deletion improves streptozotocin-induced diabetic nephropathy by directly regulating Sglt2 and Glut2. JCI Insight. 10.1172/jci.insight.163306
Mitsunori Fujino et al. (2023) Generation and mutational analysis of a transgenic murine model of the human MAF mutation. American Journal of Medical Genetics Part a. 10.1002/ajmg.a.63220
