| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human c-Myc/MYC recombinant protein (Position: N9-A439). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets myc proto-oncogene protein in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 48.8 kDa, and the supplier reports an observed band at about 65 kDa.
c-Myc (MYC) is a bHLH-leucine zipper transcription factor that pairs with MAX, binds E-box sequences and broadly amplifies the transcription of genes for growth, ribosome biogenesis, metabolism and proliferation. It also supports stem cell self-renewal and reprogramming. MYC is one of the most frequently deregulated oncogenes in human cancer.
| Target | Myc proto-oncogene protein (gene MYC; UniProt P01106, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 48.8 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Raji cells, human K562 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human c-Myc/MYC fragment (Asn9–Ala439), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places myc proto-oncogene protein in the nucleus, cytoplasm and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
MingHua Li et al. (2013) Down-Regulation of miR-129-5p Inhibits Growth and Induces Apoptosis in Laryngeal Squamous Cell Carcinoma by Targeting APC. PLoS One. 10.1371/journal.pone.0077829
Xin-Liang Xu et al. (2023) Synthesis, biological activity and mechanism of action of novel allosecurinine derivatives as potential antitumor agents. Bioorganic & Medicinal Chemistry. 10.1016/j.bmc.2023.117234
Dan Zhang et al. (2017) The role of β-catenin in the initiation and metastasis of TA2 mice spontaneous breast cancer. Journal of Cancer. 10.7150/jca.19723
Xiaoyu Li et al. (2017) Alterations in transcription and protein expressions of HCC-related genes in HepG2 cells caused by microcystin-LR. Toxicology in Vitro. 10.1016/j.tiv.2016.12.016
H. Wang et al. (2019) Long non-coding RNA LINC01503 predicts worse prognosis in glioma and promotes tumorigenesis and progression through activation of Wnt/β-catenin signaling. European Review for Medical and Pharmacological Sciences. 10.26355/eurrev_201902_17119
Sun Xiangyu et al. (2019) Aptamer-assisted superparamagnetic iron oxide nanoparticles as multifunctional drug delivery platform for chemo-photodynamic combination therapy. Journal of Materials Science-Materials in Medicine. 10.1007/s10856-019-6278-y
Junming Guo et al. (2009) Suppression of C-myc Expression Associates with Anti-Proliferation of Aloe-Emodin on Gastric Cancer Cells. Cancer Investigation. 10.1080/07357900701788130
Xue-Gong Qin et al. (2010) Effects of matrine on HepG2 cell proliferation and expression of tumor relevant proteins in vitro. Pharmaceutical Biology. 10.3109/13880200903104101
