| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-reactive protein; C-reactive protein (1-205); CRP; PTX1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human C Reactive Protein, which shares 68.8% and 53.1% amino acid (aa) sequence identity with mouse and rat C Reactive Protein, respectively. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes C-reactive protein (gene CRP) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 25.0 kDa, and the supplier reports an observed band at about 25 kDa.
C-reactive protein (CRP) is a pentameric plasma protein made by the liver whose levels rise sharply during inflammation, largely under IL-6 control. It binds phosphocholine on microbes and damaged cells in a Ca2+-dependent way and activates complement, and it is a classic marker of the acute-phase response.
| Target | C-reactive protein (gene CRP; UniProt P02741, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 25.0 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue, human liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human C Reactive Protein. Sequence identity with the mouse and rat orthologs: 68.8% and 53.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes C-reactive protein as a secreted protein. Tissue expression noted by UniProt (human): Found in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Harpreet Kaur et al. (2021) Protective effects of potential probiotic Lactobacillus rhamnosus (MTCC-5897) fermented whey on reinforcement of intestinal epithelial barrier function in a colitis-induced murine model. Food & Function. 10.1039/D0FO02641G
Sapna Balayan et al. (2024) Advancing Electrochemical Sensing: A Smart Platform for Accurate CRP Level Detection in Neonatal Septicaemia. Talanta Open. 10.1016/j.talo.2024.100369
