| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proto-oncogene c-Rel; REL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human c-Rel c-Rel contains an amino-terminal DNA-binding domain referred to as the REL homology domain (REH) and carboxy-terminal transactivation domains. The c-Rel protein is typically inhibited in unstimulated cells by I Kappa B Alpha and I Kappa B Beta . c-Rel expression is highest in hematopoietic cells with extensive research studies demonstrating its role in immune cell function and pathogenesis of disease. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADDH-18 is a rabbit monoclonal antibody that recognizes proto-oncogene c-Rel in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 68.5 kDa, and the supplier reports an observed band at about 78 kDa.
c-Rel (REL) is a member of the NF-κB family of transcription factors. It forms dimers with other NF-κB subunits and, after release from IκB inhibitors, enters the nucleus to control genes involved in immune responses, lymphocyte proliferation and survival. c-Rel is particularly important in B and T cells and is studied in immunity and lymphoma.
| Target | Proto-oncogene c-Rel (gene REL; UniProt Q04864, human) |
|---|---|
| Clone | ADDH-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 68.5 kDa |
| Observed MW | 78 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human Daudi cells, human Caco-2 cells, human A549 cells, human RT4 cells, human K562 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human c-Rel c-Rel contains an amino-terminal DNA-binding domain referred to as the REL homology domain (REH) and carboxy-terminal transactivation domains.
Reactivity Notes
The supplier lists reactivity with human. UniProt places proto-oncogene c-Rel in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
