| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement component 1 Q subcomponent-binding protein, mitochondrial; ASF/SF2-associated protein p32; Glycoprotein gC1qBP; C1qBP; Hyaluronan-binding protein 1; Mitochondrial matrix protein p32; gC1q-R protein; p33; SF2AP32; GC1QBP; HABP1; SF2P32 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human C1QBP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27C44 is a rabbit monoclonal antibody that recognizes complement component 1 Q subcomponent-binding protein, mitochondrial (gene C1QBP) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 31.4 kDa, and the supplier reports an observed band at about 36 kDa.
C1QBP (also known as p32 or gC1qR) is a mainly mitochondrial protein originally identified by its binding to the globular heads of complement C1q. It supports mitochondrial protein synthesis and has been linked to ribosome biogenesis, apoptosis, splicing and inflammation, with a cell-surface pool also reported. Researchers study it in mitochondrial function, cancer metabolism and host–pathogen interactions.
| Target | Complement component 1 Q subcomponent-binding protein, mitochondrial (gene C1QBP; UniProt Q07021, human) |
|---|---|
| Clone | 27C44 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.4 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human A549 cells, human HepG2 cells, rat small intestine tissue, rat brain tissue, mouse intestine tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human pancreas cancer tissue, rat cerebellum tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human C1QBP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places complement component 1 Q subcomponent-binding protein, mitochondrial in the mitochondrion matrix, nucleus and cell membrane. Tissue expression noted by UniProt (human): Expressed on cell surface of peripheral blood cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
