| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Scavenger receptor class B member 1;SRB1;CD36 and LIMPII analogous 1;CLA-1;CD36 antigen-like 1;Collagen type I receptor, thrombospondin receptor-like 1;SR-BI;CD36;SCARB1;CD36L1, CLA1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human C4A |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-C4A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFHB-3; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, Flow (as provided in the source record). Boster Bio Anti-C4A Monoclonal Antibody catalog # M01095-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: C4A (Scavenger receptor class B member 1).
- Antibody format: Monoclonal; clone AFHB-3; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
C4A (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as high density lipoproteins (HDL) and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins (By similarity). Necessary for selective HDL-cholesterol uptake (PubMed:26965621). Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate (By similarity). . Reported cellular localization context: Cell membrane ; Multi-pass membrane protein. Membrane, caveola; Multi-pass membrane protein. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae. Tissue expression notes (as provided): Widely expressed.
Research relevance and current trends
- Research context keywords from the source record include: Complement,Immunology,Innate Immunity.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate C4A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect C4A expression by Western blot in cell or tissue lysates, Detect C4A in FFPE tissue sections by immunohistochemistry, Quantify C4A-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 110 kDa; calculated MW: 60878 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 110 kDa
- Cellular localization (provided): Cell membrane ; Multi-pass membrane protein. Membrane, caveola; Multi-pass membrane protein. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
- Tissue details (provided): Widely expressed.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
