| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement C4-A; Acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C4A; CO4; CPAMD2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human C4A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFHB-3 is a rabbit monoclonal antibody that recognizes complement C4-A (gene C4A) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 192.8 kDa, and the supplier reports an observed band at about 193 kDa.
Complement C4-A is one of two closely related C4 isotypes in humans. Cleaved by C1s or MASP2, it releases C4b, which binds covalently to target surfaces and combines with C2a to form the classical and lectin pathway C3 convertase, and C4a, a weak anaphylatoxin. C4 copy number and levels are studied in autoimmunity and immune complex clearance.
| Target | Complement C4-A (gene C4A; UniProt P0C0L4, human) |
|---|---|
| Clone | AFHB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 192.8 kDa |
| Observed MW | 193 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human C4A.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes complement C4-A as secreted and also located in the synapse, cell projection and cell surface. Tissue expression noted by UniProt (human): Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
