| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement C5; Hemolytic complement; C5; Hc |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse C5 recombinant protein (Position: Q19-D294). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes complement C5 in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 188.9 kDa, and the supplier reports an observed band at about 75 kDa.
Complement C5 is cleaved by C5 convertases from all complement pathways into C5a and C5b. C5a is a potent anaphylatoxin and chemoattractant for neutrophils and other leukocytes, while C5b starts assembly of the membrane attack complex with C6–C9. It is widely studied in inflammation, infection and complement-mediated disease.
| Target | Complement C5 (gene C5; UniProt P06684, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 188.9 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, rat RH35 cells, mouse liver tissue, mouse RAW3647 cells, mouse ANA-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): ANA-1 cells.
Immunogen
Recombinant mouse C5 fragment (Gln19–Asp294), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes complement C5 as secreted and also located in the target cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qiong Zhang et al. (2016) Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia. Environmental Pollution. 10.1016/j.envpol.2016.07.073
