| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement C5;C5a anaphylatoxin;C5; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat C5a recombinant protein (Position: D1-R77). Rat C5a shares 62% and 82% amino acid (aa) sequences identity with human and mouse C5a, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes complement C5 in rat samples and is validated for IHC and Western blot. The predicted molecular weight is 9.0 kDa, and the supplier reports an observed band at about 115 kDa.
Complement C5 is cleaved by C5 convertases from all complement pathways into C5a and C5b. C5a is a potent anaphylatoxin and chemoattractant for neutrophils and other leukocytes, while C5b starts assembly of the membrane attack complex with C6–C9. It is widely studied in inflammation, infection and complement-mediated disease.
| Target | Complement C5 (gene C5; UniProt P08650, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 9.0 kDa |
| Observed MW | 115 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Rat) |
Samples with a confirmed band (WB): rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTAbuffer (pH 8.0).
Immunogen
Recombinant rat C5a fragment (Asp1–Arg77), expressed in E. coli. Sequence identity with the human and mouse orthologs: 62% and 82%, respectively.
Reactivity Notes
The supplier lists reactivity with rat. UniProt describes complement C5 as secreted and also located in the target cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Lu et al. (2018) C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization. Molecular Medicine Reports. 10.3892/mmr.2018.9208
