| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carbonate dehydratase XII, Carbonic anhydrase XII, Tumor antigen HOM-RCC-3.1.3, CA-XII, Carbonic anhydrase 12, CA12 |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli - derived recombinant Human CA12 (Ala25-Leu302). |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Anti-CA12 Polyclonal Antibody targets CA12 (Carbonic anhydrase XII). Commonly used in ELISA, IHC, WB workflows. Research contexts include Oncology & Angiogenesis.
Also known as: Carbonic anhydrase XII, CA12, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3, CA-XII, Carbonic anhydrase 12.
Target biology
CA12 (carbonic anhydrase XII) is a transmembrane zinc metalloenzyme that catalyzes reversible hydration of CO₂ to bicarbonate and protons, contributing to extracellular pH regulation. CA12 is expressed in multiple epithelial contexts and is frequently investigated in tumor biology where pH control supports growth and microenvironment adaptation. Common names in the literature include: Carbonic anhydrase XII, CA12, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3, CA-XII, Carbonic anhydrase 12. Typical localization reported for this class of target is plasma membrane and/or cell surface.
How this antibody helps
As a polyclonal reagent, this antibody can provide enhanced sensitivity through multi-epitope recognition. Use it to profile target expression and localization and to compare signal across conditions with matched controls.
Antigen / immunogen context
E. coli - derived recombinant Human CA12 (Ala25-Leu302).
Recommended experimental notes
- Titrate the antibody to optimize signal-to-noise for your specific sample type and detection system.
- Use appropriate positive and negative controls (e.g., target-high vs target-low samples, KO/KD where available).
- For Western blotting, include fresh protease inhibitors and confirm the dominant band at the expected molecular weight; additional bands may reflect isoforms or processing.
- For IHC, optimize fixation and antigen retrieval (buffer/pH/heat) and include isotype/secondary-only controls to assess background.
- For ELISA, optimize coating/capture conditions and validate dynamic range with appropriate standards or reference samples.
Additional notes
For research use only.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
