| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Toll-like receptor 5;Toll/interleukin-1 receptor-like protein 3;TLR5;TIL3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human CAD |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CAD antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23C35; isotype IgG; reactivity: Human. Reported application contexts include WB, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-CAD Rabbit Monoclonal Antibody catalog # M00463-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CAD (Toll-like receptor 5).
- Antibody format: Monoclonal; clone 23C35; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CAD (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. . Reported cellular localization context: Membrane ; Single-pass type I membrane protein . Tissue expression notes (as provided): Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Research relevance and current trends
- Research context keywords from the source record include: Immunoglobulins,Immunology,Innate Immunity,Macrophage/Inflammation,Receptors,TLR Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate CAD antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CAD expression by Western blot in cell or tissue lysates, Localize CAD by immunofluorescence/immunocytochemistry in cultured cells, Quantify CAD-positive cells by flow cytometry in single-cell suspensions, Enrich CAD by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 260 kDa; calculated MW: 97834 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 260 kDa
- Cellular localization (provided): Membrane ; Single-pass type I membrane protein .
- Tissue details (provided): Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
