Anti-Cadherin 10 Rabbit Monoclonal Antibody

SKU:BHA21010375
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Boster Bio
Boster Bio
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Overview
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Anti-CDH10 antibody (Rabbit monoclonal, clone 28C85, IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes cadherin-10. Observed band in Western blot: about 140 kDa.
Target CDH10
clone number 28C85
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M10616 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB
      • Application details: WB 1:500-2000
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target CDH10
Alternative names Cadherin-10; T2-cadherin; CDH10
Gene ID 1008
UniProt # Q9Y6N8
Host Rabbit
Clone 28C85
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human Cadherin 10
Molecular weight 88.5 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cell membrane; Single-pass type I membrane protein.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M10616
Main SKU BHA21010375
Rabbit Monoclonal

Product Overview

Clone 28C85 is a rabbit monoclonal antibody that recognizes cadherin-10 (gene CDH10) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 88.5 kDa, and the supplier reports an observed band at about 140 kDa.

Cadherin-10 (CDH10) is a type II classical cadherin that mediates calcium-dependent, homophilic cell–cell adhesion. It is expressed mainly in the brain, where it is thought to take part in neural development and synapse organization.

Target Cadherin-10 (gene CDH10; UniProt Q9Y6N8, human)
Clone 28C85
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 88.5 kDa
Observed MW 140 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): U-87 MG cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human cadherin 10.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places cadherin-10 in the cell membrane. Tissue expression noted by UniProt (human): Predominantly expressed in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 88.5 kDa; observed band at about 140 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human cadherin 10.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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