| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform;3.1.3.16;CAM-PRP catalytic subunit;Calmodulin-dependent calcineurin A subunit alpha isoform;PPP3CA;CALNA, CNA; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Calcineurin A |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PPP3CA antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABOG-16; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-Calcineurin A Rabbit Monoclonal Antibody catalog # M03026. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PPP3CA (Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform).
- Antibody format: Monoclonal; clone ABOG-16; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PPP3CA (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. . Reported cellular localization context: Cell membrane . Cell membrane, sarcolemma . Nucleus . Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. . Tissue expression notes (as provided): Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cancer Metabolism,Cardiovascular,Epigenetics and Nuclear Signaling,Hypoxia,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate PPP3CA antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PPP3CA expression by Western blot in cell or tissue lysates, Localize PPP3CA by immunofluorescence/immunocytochemistry in cultured cells, Quantify PPP3CA-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 59 kDa; calculated MW: 58688 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 59 kDa
- Cellular localization (provided): Cell membrane . Cell membrane, sarcolemma . Nucleus . Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. .
- Tissue details (provided): Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
