| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform;3.1.3.16;CAM-PRP catalytic subunit;Calmodulin-dependent calcineurin A subunit alpha isoform;PPP3CA;CALNA, CNA; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Calcineurin A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABOG-16 is a rabbit monoclonal antibody that recognizes protein phosphatase 3 catalytic subunit alpha (gene PPP3CA) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 58.7 kDa, and the supplier reports an observed band at about 59 kDa.
Calcineurin Aα (PPP3CA) is the main catalytic subunit of calcineurin, a Ca2+/calmodulin-activated serine/threonine phosphatase. Calcineurin dephosphorylates NFAT transcription factors to drive T-cell activation and other responses, and it is the target of cyclosporin A and tacrolimus. It is studied in immunity, neurons and cardiac hypertrophy.
| Target | Protein phosphatase 3 catalytic subunit alpha (gene PPP3CA; UniProt Q08209, human) |
|---|---|
| Clone | ABOG-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.7 kDa |
| Observed MW | 59 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human MCF-7 cells, human U2OS cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human calcineurin A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein phosphatase 3 catalytic subunit alpha in the cytoplasm, cell membrane and cell projection. Tissue expression noted by UniProt (human): Expressed in keratinocytes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiali Nie et al. (2018) Ranolazine prevents pressure overload-induced cardiac hypertrophy and heart failure by restoring aberrant Na+ and Ca2+ handling. Journal of Cellular Physiology. 10.1002/jcp.27791
Lu-yi-fei Li et al. (2020) Sacubitril/valsartan attenuates atrial electrical and structural remodelling in a rabbit model of atrial fibrillation. European Journal of Pharmacology. 10.1016/j.ejphar.2020.173120
