| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calcitonin; Calca; Calc |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Calcitonin, which shares 90.6% and 96.9% amino acid (aa) sequence identity with human and rat Calcitonin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets calcitonin (gene Calca) in human, mouse and rat samples and is validated for IHC. The predicted molecular weight is 15.1 kDa.
CALCA encodes calcitonin gene-related peptide 1 (αCGRP), produced by alternative splicing of the calcitonin gene in sensory and central neurons. CGRP is a powerful vasodilator acting through the CALCRL–RAMP1 receptor and a key mediator of neurogenic inflammation and pain signaling. It is a major target in migraine research.
| Target | Calcitonin (gene Calca; UniProt P70160, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 15.1 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of mouse calcitonin. Sequence identity with the human and rat orthologs: 90.6% and 96.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes calcitonin as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ji-Hong Yang et al. (2013) STZ treatment induced apoptosis of retinal cells and effect of up-regulation of calcitonin gene related peptide in rats. Journal of Diabetes and Its Complications. 10.1016/j.jdiacomp.2013.08.001
Jun-jun Fan et al. (2014) Different Effects of Implanting Sensory Nerve or Blood Vessel on the Vascularization, Neurotization, and Osteogenesis of Tissue-Engineered Bone In Vivo. Biomed Research International. 10.1155/2014/412570
