| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calreticulin;CRP55;Calregulin;Endoplasmic reticulum resident protein 60;ERp60;HACBP;grp60;CALR;CRTC; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Calreticulin - ER Marker |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CGO-3 is a rabbit monoclonal antibody that recognizes calreticulin in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 48.1 kDa, and the supplier reports an observed band at about 60 kDa.
Calreticulin (CALR) is an abundant Ca2+-binding lectin chaperone of the endoplasmic reticulum. Together with calnexin, it folds and quality-checks newly made glycoproteins, and it also stores ER calcium and helps load peptides onto MHC class I. Calreticulin exposed on the cell surface acts as an "eat me" signal, and CALR mutations drive some myeloproliferative neoplasms.
| Target | Calreticulin (gene CALR; UniProt P27797, human) |
|---|---|
| Clone | CGO-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.1 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human COLO320 cells, human HL-60 cells, human HeLa cells, human MCF-7 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat cerebral cortex tissue, human renal cancer tissue, human placenta tissue, mouse testis tissue, mouse cerebellum tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human calreticulin - ER Marker.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places calreticulin in the endoplasmic reticulum lumen, cytoplasm, cell surface and sarcoplasmic reticulum lumen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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