| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calcium/calmodulin-dependent protein kinase type II subunit delta;CaM kinase II subunit delta;CaMK-II subunit delta;2.7.11.17;CAMK2D;CAMKD; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CaMKII delta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABBF-3 is a rabbit monoclonal antibody that recognizes calcium/calmodulin-dependent protein kinase type II subunit delta (gene CAMK2D) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 56.4 kDa, and the supplier reports an observed band at about 50 kDa.
CaMKIIδ (CAMK2D) is the main CaMKII isoform in the heart. It phosphorylates ion channels, phospholamban and ryanodine receptors to tune Ca2+ handling and excitation–contraction coupling in cardiomyocytes. Excess CaMKIIδ activity is linked to cardiac hypertrophy, heart failure and arrhythmia, making it a major cardiac research target.
| Target | Calcium/calmodulin-dependent protein kinase type II subunit delta (gene CAMK2D; UniProt Q13557, human) |
|---|---|
| Clone | ABBF-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.4 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Samples with a confirmed band (WB): human Daudi cells, human U-87MG cells, human HUVEC cells, human A549 cells, rat C6 cells, mouse MH-S cells, mouse Neuro-2a cells, mouse RAW264.7 cells, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (ICC/IF): mouse MH-S cells.
Immunogen
Synthetic peptide based on the sequence of human caMKII delta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places calcium/calmodulin-dependent protein kinase type II subunit delta in the cell membrane and sarcoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed in cardiac muscle and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
