| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein 90 homolog, Hsp90 |
| Accession number | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli - derived recombinant Candida albicans HSP90 (Met1-Asp707). |
| Conjugate | |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Anti-Candida albicans HSP90 Polyclonal Antibody is an affinity-purified rabbit polyclonal antibody raised against HSP90 (heat shock protein 90 homolog) of Candida albicans. It is supplied unconjugated in liquid form and is listed by the manufacturer for ELISA, immunohistochemistry and Western blot.
Hsp90 is an essential, ATP-dependent molecular chaperone that promotes the maturation, structural maintenance and regulation of client proteins involved in cell cycle control and signal transduction. In C. albicans the chaperone additionally governs the yeast-to-hypha morphogenetic switch and underpins tolerance to azole and echinocandin antifungals, which is why fungal Hsp90 is studied both as a virulence determinant and as an antifungal target. Hsp90 is also an immunodominant antigen during invasive candidiasis - it is the target of the experimental anti-Hsp90 antibody fragment efungumab (Mycograb), which was investigated as an antifungal adjunct - so reagents that detect the fungal chaperone are used in seroreactivity, host-pathogen interaction and antifungal mechanism studies.
Applications
| Application | Manufacturer dilution range |
|---|---|
| ELISA | 1:5,000-1:20,000 |
| Immunohistochemistry (IHC) | 1:50-1:500 |
| Western blot (WB) | 1:500-1:2,000 |
Dilutions are manufacturer starting points. Titrate for each sample type, fixation method and detection system, and run an uninfected or fungus-free control alongside the test sample.
Specifications
| Host species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species reactivity | Candida albicans |
| Target | HSP90 |
| Alternative names | Heat shock protein 90 homolog, Hsp90 |
| Immunogen | E. coli - derived recombinant Candida albicans HSP90 (Met1-Asp707). |
| Purification | Purified by antigen affinity column. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% ProClin 300. Please refer to the hardcopy datasheet or the lot-specific COA supplied with the product for buffer details. |
| Accession | P46598 |
| Stability and storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
Immunogen & Antigen
The antiserum was raised against an E. coli-derived recombinant C. albicans HSP90 spanning Met1–Asp707, essentially the full-length chaperone, and was then purified on an antigen affinity column - the antibody pool is therefore enriched for antibodies recognising epitopes distributed across the whole chaperone rather than a single domain.
The manufacturer’s Western blot figure was run against the matching recombinant antigen, not a native fungal lysate, so the band position reflects that recombinant construct. Confirm the apparent size in your own system before using it as an identity criterion.
Reactivity Notes
Manufacturer-stated reactivity is limited to Candida albicans. Reactivity with C. auris, C. glabrata or other Candida species is not claimed and should be confirmed in-house before use in a cross-species comparison.
Hsp90 is one of the most highly conserved proteins in eukaryotes, so a polyclonal raised against the full-length fungal chaperone can in principle recognise host Hsp90 as well. Cross-reactivity with host Hsp90 is not characterised, so for infected-tissue IHC or lysate Western blots include an uninfected host sample as a control before attributing signal to fungal Hsp90.
Safety & Handling
The formulation contains 0.05% ProClin 300 as a preservative — handle with gloves, avoid skin and eye contact, and consult the linked SDS. The 50% glycerol content keeps the product liquid at -20°C, so working aliquots can be drawn without thawing; for -80°C storage, aliquot first and avoid repeated freeze-thaw cycles.
For research use only. Not for use in diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
