| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adenylyl cyclase-associated protein 1;CAP 1;CAP1;CAP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CAP1 recombinant protein (Position: A2-R123). Human CAP1 shares 92.6% and 93.4% amino acid (aa) sequence identity with mouse and rat CAP1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes adenylyl cyclase-associated protein 1 (gene CAP1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 51.9 kDa, and the supplier reports an observed band at about 55–60 kDa.
Adenylyl cyclase-associated protein 1 (CAP1) is a conserved actin-binding protein that regulates actin filament turnover. Working with cofilin, it promotes filament disassembly and recycles actin monomers, and it has been implicated in cell polarity, migration and adhesion. It is studied in cytoskeletal dynamics and in cancer cell motility.
| Target | Adenylyl cyclase-associated protein 1 (gene CAP1; UniProt Q01518, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 51.9 kDa |
| Observed MW | 55–60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HeLa cells, rat thymus tissue, rat spleen tissue, mouse thymus tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human CAP1 fragment (Ala2–Arg123), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 92.6% and 93.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places adenylyl cyclase-associated protein 1 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
