| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Adenylyl cyclase-associated protein 1;CAP 1;CAP1;CAP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human CAP1 recombinant protein (Position: A2-R123). Human CAP1 shares 92.6% and 93.4% amino acid (aa) sequence identity with mouse and rat CAP1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CAP1 (Adenylyl cyclase-associated protein 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-CAP1 Antibody Picoband® catalog # PB9923. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human CAP1 recombinant protein (Position: A2-R123). Human CAP1 shares 92.6% and 93.4% amino acid (aa) sequence identity with mouse and rat CAP1, respectively. (reported region: A2-R123).
- Molecular weight context: reported MW: 60 kDa; calculated MW: 51901 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Adenylyl cyclase-associated protein 1; Adenylyl cyclase-associated protein 1. Adenylyl cyclase-associated protein 1 is an enzyme that in humans is encoded by the CAP1 gene. This gene is mapped to 1p34.2. The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. And the human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified. Functional note: ly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity. Reported localization: Cell membrane ; Peripheral membrane protein . Expression/tissue context: High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Research relevance and current trends
- Cytoskeleton/ECM: Researchers commonly examine how CAP1 (Adenylyl cyclase-associated protein 1) relates to this theme using model systems and orthogonal readouts.
- DNA/RNA: Researchers commonly examine how CAP1 (Adenylyl cyclase-associated protein 1) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how CAP1 (Adenylyl cyclase-associated protein 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CAP1 (Adenylyl cyclase-associated protein 1) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
