| Field | Specification |
|---|---|
| Target | |
| Alternative names | Calpain-5; Calpain htra-3; New calpain 3; nCL-3; CAPN5; NCL3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CAPN5 recombinant protein (Position: N583-N616). Human CAPN5 shares 84.8% and 78.8% amino acid (aa) sequence identity with mouse and rat CAPN5, respectively. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets calpain-5 (gene CAPN5) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
Calpain-5 is a Ca2+-regulated cysteine protease of the calpain family that lacks the typical C-terminal EF-hand domain. It is expressed in the retina, brain and other tissues, and mutations in CAPN5 cause an inherited inflammatory eye disease (autosomal dominant neovascular inflammatory vitreoretinopathy).
| Target | Calpain-5 (gene CAPN5; UniProt O15484, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human CAPN5 fragment (Asn583–Asn616), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 84.8% and 78.8%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Expressed in many tissues.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
