| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carboxypeptidase B2;3.4.17.20;Carboxypeptidase R;CPR;Carboxypeptidase U;CPU;Thrombin-activable fibrinolysis inhibitor;TAFI;Cpb2;Tafi; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse CPB2 recombinant protein (Position: K165-D387). Mouse CPB2 shares 86.1% and 96.9% amino acid (aa) sequence identity with human and rat CPB2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes carboxypeptidase B2 (gene Cpb2) in mouse samples and is validated for Western blot. The predicted molecular weight is 48.9 kDa, and the supplier reports an observed band at about 48 kDa.
Carboxypeptidase B2 (CPB2), also called TAFI, is a plasma carboxypeptidase activated by the thrombin–thrombomodulin complex. It removes C-terminal lysines from partially degraded fibrin, which slows plasminogen binding and fibrinolysis, and it also inactivates bradykinin and anaphylatoxins, linking coagulation and inflammation.
| Target | Carboxypeptidase B2 (gene Cpb2; UniProt Q9JHH6, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 48.9 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|
Samples with a confirmed band (WB): mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse CPB2 fragment (Lys165–Asp387), expressed in E. coli. Sequence identity with the human and rat orthologs: 86.1% and 96.9%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes carboxypeptidase B2 as a secreted protein. Tissue expression noted by UniProt (mouse): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
