| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleotide-binding oligomerization domain-containing protein 1;Caspase recruitment domain-containing protein 4;NOD1;CARD4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CARD4 recombinant protein (Position: M1-M160). Human CARD4 shares 82% amino acid (aa) sequence identity with mouse CARD4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes nucleotide-binding oligomerization domain-containing protein 1 (gene NOD1) in human and rat samples and is validated for IHC, ICC/IF and Western blot. The predicted molecular weight is 107.7 kDa, and the supplier reports an observed band at about 107 kDa.
NOD1 is a cytosolic pattern recognition receptor that detects iE-DAP, a peptidoglycan fragment found mainly in Gram-negative bacteria. Once activated it recruits the kinase RIPK2 to trigger NF-κB and MAPK signaling and inflammatory cytokine production. NOD1 is studied in innate immunity, host–microbe interactions and inflammatory disease.
| Target | Nucleotide-binding oligomerization domain-containing protein 1 (gene NOD1; UniProt Q9Y239, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 107.7 kDa |
| Observed MW | 107 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL |
|---|---|
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| Western blot | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): A549 cells, rat cardiac muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): A549 cells, HeLa cells, SMMC-7721 cells.
Immunogen
Recombinant human CARD4 fragment (Met1–Met160), expressed in E. coli. Sequence identity with the mouse ortholog: 82%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places nucleotide-binding oligomerization domain-containing protein 1 in the cell membrane, apical cell membrane, basolateral cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiande Ma et al. (2020) Nucleotide-binding oligomerization domain protein 1 enhances oxygen-glucose deprivation and reperfusion injury in cortical neurons via activation of endoplasmic reticulum stress-mediated autophagy. Experimental and Molecular Pathology. 10.1016/j.yexmp.2020.104525
