Anti-Cardiac Troponin C Rabbit Monoclonal Antibody

SKU:BHA21009569
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-TNNC1 antibody (Rabbit monoclonal, clone 20T71, IgG). Validated for Western blot and immunohistochemistry. Reactivity: Human, Mouse, Rat. Recognizes troponin C, slow skeletal and cardiac muscles.
Target TNNC1
clone number 20T71
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB, IHC
Options selector
Catalog no. Size Conjugation
M03153-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC
      Application details: WB 1:500-2000<br>IHC 1:50-200<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target TNNC1
Alternative names Troponin C, slow skeletal and cardiac muscles; TN-C; TNNC1; TNNC
Gene ID 7134
UniProt # P63316
Host Rabbit
Clone 20T71
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
Immunogen A synthesized peptide derived from human Cardiac Troponin C
Molecular weight 18.4 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Membrane, coated pit ; Peripheral membrane protein ; Cytoplasmic side . Melanosome . Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M03153-1
Main SKU BHA21009569
Rabbit Monoclonal

Product Overview

Clone 20T71 is a rabbit monoclonal antibody that recognizes troponin C, slow skeletal and cardiac muscles (gene TNNC1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 18.4 kDa, and the supplier reports an observed band at about 18 kDa.

Troponin C (TNNC1) is the calcium-binding subunit of the troponin complex in cardiac and slow skeletal muscle. When calcium binds troponin C, it relieves the inhibition exerted by troponin I on actin–myosin interaction, allowing muscle contraction. TNNC1 is studied in cardiac muscle physiology and in inherited cardiomyopathies.

Target Troponin C, slow skeletal and cardiac muscles (gene TNNC1; UniProt P63316, human)
Clone 20T71
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 18.4 kDa
Observed MW 18 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200

Immunogen

Synthetic peptide based on the sequence of human cardiac Troponin C.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 18.4 kDa; observed band at about 18 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human cardiac Troponin C.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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