| Field | Specification |
|---|---|
| Target | |
| Alternative names | Troponin C, slow skeletal and cardiac muscles;TN-C;TNNC1;TNNC; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human cardiac Troponin C, identical to the related rat and mouse sequences . |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects troponin C, slow skeletal and cardiac muscles (gene TNNC1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 18.4 kDa, and the supplier reports an observed band at about 18 kDa.
Troponin C (TNNC1) is the calcium-binding subunit of the troponin complex in cardiac and slow skeletal muscle. When calcium binds troponin C, it relieves the inhibition exerted by troponin I on actin–myosin interaction, allowing muscle contraction. TNNC1 is studied in cardiac muscle physiology and in inherited cardiomyopathies.
| Target | Troponin C, slow skeletal and cardiac muscles (gene TNNC1; UniProt P63316, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 18.4 kDa |
| Observed MW | 18 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat, Mouse) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Rat, Human, Mouse) |
Samples with a confirmed band (WB): rat skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): rat cardiac muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human cardiac Troponin C. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chengbao Li et al. (2020) An improved two-step method for extraction and purification of primary cardiomyocytes from neonatal mice. Journal of Pharmacological and Toxicological Methods. 10.1016/j.vascn.2020.106887
