| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone-arginine methyltransferase CARM1; Coactivator-associated arginine methyltransferase 1; Protein arginine N-methyltransferase 4; CARM1; PRMT4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CARM1 recombinant protein (Position: D65-H385). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes histone-arginine methyltransferase CARM1 in human samples and is validated for Western blot and ELISA. The predicted molecular weight is 65.9 kDa, and the supplier reports an observed band at about 66 kDa.
CARM1 (also called PRMT4) is a protein arginine methyltransferase that adds asymmetric dimethyl marks to arginines. It methylates histone H3 at Arg17 and many non-histone proteins, acting as a transcriptional coactivator with p300/CBP and nuclear receptors and influencing splicing and mRNA stability. It is studied in gene regulation, development and cancer.
| Target | Histone-arginine methyltransferase CARM1 (gene CARM1; UniProt Q86X55, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 65.9 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human MCF-7 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human CARM1 fragment (Asp65–His385), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places histone-arginine methyltransferase CARM1 in the nucleus, cytoplasm and chromosome. Tissue expression noted by UniProt (human): Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
