| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-S1-casein; CSN1S1; CASA; CSN1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Casein |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22C70 is a rabbit monoclonal antibody that recognizes alpha-S1-casein (gene CSN1S1) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 21.7 kDa, and the supplier reports an observed band at about 22 kDa.
Alpha-S1-casein (CSN1S1) is a major milk phosphoprotein that assembles with other caseins into micelles, allowing milk to carry large amounts of calcium phosphate. Peptides released from it during digestion can have biological activity, and caseins are studied in lactation biology, mammary gland function and dairy science.
| Target | Alpha-S1-casein (gene CSN1S1; UniProt P47710, human) |
|---|---|
| Clone | 22C70 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.7 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human milk.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human casein.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes alpha-S1-casein as a secreted protein. Tissue expression noted by UniProt (human): Mammary gland specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
