| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-1;CASP-1;3.4.22.36;Interleukin-1 beta convertase;IL-1BC;Interleukin-1 beta-converting enzyme;ICE;IL-1 beta-converting enzyme;p45;Caspase-1 subunit p20;Caspase-1 subunit p10;CASP1;IL1BC, IL1BCE; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from Caspase-1 + p10 + p12 |
| Molecular weight | |
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| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
Clone 26C50 is a rabbit monoclonal antibody that recognizes caspase-1 (gene CASP1) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 45.2 kDa, and the supplier reports an observed band at about 45 kDa.
Caspase-1 is the inflammatory caspase activated by canonical inflammasomes such as NLRP3, NLRC4 and AIM2. Once active, it processes pro-IL-1β and pro-IL-18 into mature cytokines and cleaves gasdermin D to trigger pyroptosis, a lytic, inflammatory form of cell death. It is a central readout in innate immunity and inflammation research.
| Target | Caspase-1 (gene CASP1; UniProt P29466, human) |
|---|---|
| Clone | 26C50 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.2 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of caspase-1 + p10 + p12.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places caspase-1 in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expressed in larger amounts in spleen and lung.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lingzhi Huang et al. (2025) Elevation of ISG15 promotes diabetic kidney disease by modulating renal tubular epithelial cell pyroptosis. Clinical and Translational Medicine. 10.1002/ctm2.70337
Jingjing Liu et al. (2021) Terazosin Stimulates Pgk1 to Remedy Gastrointestinal Disorders. International Journal of Molecular Sciences. 10.3390/ijms23010416
Zhang Yani et al. (2025) Huanglian Jiedu Decoction improves the"central-peripheral"inflammatory microenvironment and enhances the cognitive function of APP/PS1 mice by inhibiting the activation of NLRP3 inflammasome mediated by gut microbiota. Chinese Medicine. 10.1186/s13020-025-01180-4
Zhang Qiuyue et al. (2017) Dexmedetomidine Alleviates Hyperoxia-Induced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation. Cellular Physiology and Biochemistry. 10.1159/000479609
Yuan Jiang et al. (2024) Methyltransferase‐Like 3–Mediated N6‐Methyladenosine RNA Methylation Regulates Hypoxia‐Induced Pulmonary Arterial Smooth Muscle Cell Pyroptosis by Targeting PTEN. Journal of the American Heart Association. 10.1161/JAHA.124.034470
Ming-Wei Fan et al. (2026) Electroacupuncture protects gastric Cajal cells by reducing macrophage pyroptosis in diabetic gastroparesis. World Journal of Diabetes. 10.4239/wjd.v17.i2.114252
Haojiang Duan et al. (2024) GBP1 promotes acute rejection after liver transplantation by inducing Kupffer cells pyroptosis. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2024.167644
Jing Huang et al. (2024) SIRT5 safeguards against T-2 toxin induced liver injury by repressing iron accumulation, oxidative stress, and the activation of NLRP3 inflammasome. Toxicology and Applied Pharmacology. 10.1016/j.taap.2024.117084
Luodong Yang et al. (2024) Stress-induced NLRP3 inflammasome activation and myelin alterations in the hippocampus of PTSD rats. Neuroscience. 10.1016/j.neuroscience.2024.07.028
Kuang Peng et al. (2015) P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation. International Journal of Molecular Medicine. 10.3892/ijmm.2015.2129
