| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-1;CASP-1;3.4.22.36;Interleukin-1 beta convertase;IL-1BC;Interleukin-1 beta-converting enzyme;ICE;IL-1 beta-converting enzyme;p45;Caspase-1 subunit p20;Caspase-1 subunit p10;CASP1;IL1BC, IL1BCE; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-1(P10). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes caspase-1 (gene CASP1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 45.2 kDa, and the supplier reports an observed band at about 45 kDa.
Caspase-1 is the inflammatory caspase activated by canonical inflammasomes such as NLRP3, NLRC4 and AIM2. Once active, it processes pro-IL-1β and pro-IL-18 into mature cytokines and cleaves gasdermin D to trigger pyroptosis, a lytic, inflammatory form of cell death. It is a central readout in innate immunity and inflammation research.
| Target | Caspase-1 (gene CASP1; UniProt P29466, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 45.2 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): MCF-7 cells, HeLa cells, SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the C-terminal region of human caspase-1(P10).
Reactivity Notes
The supplier lists reactivity with human. UniProt places caspase-1 in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Expressed in larger amounts in spleen and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jingjing Liu et al. (2021) Terazosin Stimulates Pgk1 to Remedy Gastrointestinal Disorders. International Journal of Molecular Sciences. 10.3390/ijms23010416
Peng Yuan et al. (2021) Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway. Phytomedicine. 10.1016/j.phymed.2021.153555
Xuening Pang et al. (2021) Xanthoangelol modulates Caspase-1-dependent pyroptotic death among hepatocellular carcinoma cells with high expression of GSDMD. Journal of Functional Foods. 10.1016/j.jff.2021.104577
Yuefei Jin et al. (2016) Involvement of EGF receptor signaling and NLRP12 inflammasome in fine particulate matter-induced lung inflammation in mice. Environmental Toxicology. 10.1002/tox.22308
Du Jingwei et al. (2023) RBCK1 Overexpression Attenuates Inflammation and Mobility of Derp1-Induced Nasal Mucosal Cells by Downregulating NLRP3. International Archives of Allergy and Immunology. 10.1159/000527962
Shuyin Duan et al. (2019) NLRP3 inflammasome activation involved in LPS and coal tar pitch extract-induced malignant transformation of human bronchial epithelial cells. Environmental Toxicology. 10.1002/tox.22725
Feng Zhang et al. (2016) Ligustrazine disrupts lipopolysaccharide-activated NLRP3 inflammasome pathway associated with inhibition of Toll-like receptor 4 in hepatocytes. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2016.01.018
