| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-2;CASP-2;3.4.22.55;Neural precursor cell expressed developmentally down-regulated protein 2;NEDD-2;Protease ICH-1;Caspase-2 subunit p18;Caspase-2 subunit p13;Caspase-2 subunit p12;CASP2;ICH1, NEDD2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Caspase-2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DGC-3 is a rabbit monoclonal antibody that recognizes caspase-2 (gene CASP2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 50.7 kDa; the supplier reports observed bands at 48, 30, 12 kDa.
Caspase-2 is a highly conserved initiator caspase that can be activated in the PIDDosome, a complex formed with PIDD1 and RAIDD (CRADD). It responds to cellular stresses such as DNA damage and mitotic errors, and it can cleave BID to engage mitochondrial apoptosis. It is studied as a guardian of genome stability and in tumor suppression.
| Target | Caspase-2 (gene CASP2; UniProt P42575, human) |
|---|---|
| Clone | DGC-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 50.7 kDa |
| Observed MW | 48, 30, 12 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with confirmed staining (IHC): mouse testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human caspase-2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
