| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;CASP3;CPP32; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Caspase-3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOE-3 is a rabbit monoclonal antibody that recognizes caspase-3 (gene CASP3) in human samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 31.6 kDa, and the supplier reports an observed band at about 32 kDa.
Caspase-3 is the major executioner caspase of apoptosis. Activated by initiator caspases such as caspase-8 and caspase-9, it cleaves hundreds of cellular proteins, including PARP1, leading to the typical features of apoptotic cell death. Cleaved (active) caspase-3 is one of the most widely used apoptosis markers in Western blot and immunohistochemistry.
| Target | Caspase-3 (gene CASP3; UniProt P42574, human) |
|---|---|
| Clone | AOE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.6 kDa |
| Observed MW | 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human HepG2 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human caspase-3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places caspase-3 in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in lung, spleen, heart, liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haoyu Chen et al. (2024) “Butterfly-effect” nano-platforms for cascade immune amplification and tumor clearance through multi-modal therapy and immune sensitization. Chemical Engineering Journal. 10.1016/j.cej.2024.153103
Wenhua Chen et al. (2024) Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2024.116276
Qing Zhang et al. (2022) Glabridin from Glycyrrhiza glabra Possesses a Therapeutic Role against Keloid via Attenuating PI3K/Akt and Transforming Growth Factor-β1/SMAD Signaling Pathways. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.2c02045
Lu Kong et al. (2019) Molecular mechanisms underlying nickel nanoparticle induced rat Sertoli-germ cells apoptosis. Science of the Total Environment. 10.1016/j.scitotenv.2019.07.107
Zhong Gang et al. (2018) Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway. Cellular Physiology and Biochemistry. 10.1159/000495926
Gang Yang et al. (2024) HNF1A induces glioblastoma by upregulating EPS8 and activating PI3K/AKT signaling pathway. Biochemical Pharmacology. 10.1016/j.bcp.2024.116133
Shaohua Zhai et al. (2025) Echinococcus granulosus-Induced Liver Damage Through Ferroptosis in Rat Model. Cells. 10.3390/cells14050328
Alhomedy M. Alharbi et al. (2024) Saroglitazar ameliorates 5- Fluorouracil-induced hepatorenal damage in rats. International Immunopharmacology. 10.1016/j.intimp.2024.113407
Lei Jiang et al. (2019) Sonodynamic therapy in atherosclerosis by curcumin nanosuspensions: Preparation design, efficacy evaluation, and mechanisms analysis. European Journal of Pharmaceutics and Biopharmaceutics. 10.1016/j.ejpb.2019.12.005
Yong He et al. (2023) Rutaecarpine Ameliorates Murine N-Methyl-N’-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis by Sonic Hedgehog Pathway. Molecules. 10.3390/molecules28176294
