| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-4;CASP-4;3.4.22.57;ICE (rel)-II;Protease ICH-2;Protease TX;Caspase-4 subunit 1;Caspase-4 subunit 2;CASP4;ICH2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase 4. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets caspase-4 (gene CASP4) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 43.3 kDa, and the supplier reports an observed band at about 20 kDa.
Caspase-4 is a human inflammatory caspase that directly senses cytosolic lipopolysaccharide from Gram-negative bacteria. Binding LPS activates it to cleave gasdermin D, triggering pyroptosis through the non-canonical inflammasome and indirectly activating NLRP3. It is studied in innate immunity, sepsis and ER stress, and its mouse functional counterpart is caspase-11.
| Target | Caspase-4 (gene CASP4; UniProt P49662, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 43.3 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): MCF-7 cells, HeLa cells, Jurkat cells, CEM cells, SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the N-terminal region of human caspase 4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places caspase-4 in the cytoplasm, endoplasmic reticulum membrane and mitochondrion. Tissue expression noted by UniProt (human): Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
