| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-7;CASP-7;3.4.22.60;Apoptotic protease Mch-3;Cysteine protease LICE2;Caspase-7 subunit p20;Caspase-7 subunit p11;Casp7;Lice2, Mch3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Caspase-7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EEB-3 is a rabbit monoclonal antibody that recognizes caspase-7 (gene Casp7) in mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 34.1 kDa, and the supplier reports an observed band at about 34 kDa.
Caspase-7 is an executioner caspase that, like caspase-3, is activated by initiator caspases and cleaves proteins at DEVD-type motifs during apoptosis. It shares many substrates with caspase-3, including PARP1, and also acts in pyroptosis and inflammation. Cleaved caspase-7 is commonly used as an apoptosis marker.
| Target | Caspase-7 (gene Casp7; UniProt P97864, mouse) |
|---|---|
| Clone | EEB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.1 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human caspase-7.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes caspase-7 as secreted and also located in the cytoplasm and nucleus. Tissue expression noted by UniProt (mouse): Highly expressed in heart, lung, liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
