| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-7;CASP-7;3.4.22.60;Apoptotic protease Mch-3;CMH-1;ICE-like apoptotic protease 3;ICE-LAP3;Caspase-7 subunit p20;Caspase-7 subunit p11;CASP7;MCH3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7(P11), different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets caspase-7 (gene CASP7) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 34.3 kDa, and the supplier reports an observed band at about 34 kDa.
Caspase-7 is an executioner caspase that, like caspase-3, is activated by initiator caspases and cleaves proteins at DEVD-type motifs during apoptosis. It shares many substrates with caspase-3, including PARP1, and also acts in pyroptosis and inflammation. Cleaved caspase-7 is commonly used as an apoptosis marker.
| Target | Caspase-7 (gene CASP7; UniProt P55210, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 34.3 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
Samples with a confirmed band (WB): HeLa cells, MCF-7 cells, rat liver tissue, rat kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide from the middle region of human caspase-7(P11). It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes caspase-7 as secreted and also located in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
