| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-8;CASP-8;3.4.22.61;Apoptotic cysteine protease;Apoptotic protease Mch-5;CAP4;FADD-homologous ICE/ced-3-like protease;FADD-like ICE;FLICE;ICE-like apoptotic protease 5;MORT1-associated ced-3 homolog;MACH;Caspase-8 subunit p18;Caspase-8 subunit p10;CASP8;MCH5; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Caspase-8 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EEG-3 is a rabbit monoclonal antibody that recognizes caspase-8 (gene CASP8) in human samples and is validated for Western blot. The predicted molecular weight is 55.4 kDa, and the supplier reports an observed band at about 50 kDa.
Caspase-8 is the initiator caspase of the extrinsic apoptosis pathway. Recruited through FADD to death receptors such as Fas and TNFR1, it becomes active and cleaves caspase-3, caspase-7 and BID. Caspase-8 also restrains necroptosis by cleaving RIPK1 and RIPK3, which makes it a central switch between apoptosis, necroptosis and inflammation.
| Target | Caspase-8 (gene CASP8; UniProt Q14790, human) |
|---|---|
| Clone | EEG-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.4 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human Caco-2 cells, human K562 cells, human RT4 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human caspase-8.
Reactivity Notes
The supplier lists reactivity with human. UniProt places caspase-8 in the cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (human): Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan-Qing Guan et al. (2011) Pathway of programmed cell death in HeLa cells induced by polymeric anti-cancer drugs. Biomaterials. 10.1016/j.biomaterials.2011.01.060
Shen Jing et al. (2018) Sinoporphyrin Sodium-Mediated Sonodynamic Therapy Inhibits RIP3 Expression and Induces Apoptosis in the H446 Small Cell Lung Cancer Cell Line. Cellular Physiology and Biochemistry. 10.1159/000496045
Jing-Jing Xing et al. (2019) Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice. Antioxidants. 10.3390/antiox8090347
Ruyi Xie et al. (2025) Rufy3 Knockdown Induces PANoptosis Through MAP4/CDK1 Axis to Inhibit Colorectal Cancer Growth: Evidence From In Vitro and In Vivo Models. FASEB Journal. 10.1096/fj.202501217R
Mi Xiao-jie et al. (2018) The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways. Scientific Reports. 10.1038/s41598-018-34156-6
Wang Qirong et al. (2024) The Study of SRSF1 Regulates Abnormal Alternative Splicing of BCL2L11 and the Role in Refractory Acute Myeloid Leukemia. Chemotherapy. 10.1159/000539414
Xiaoxue Wu et al. (2018) Resveratrol induces apoptosis in SGC‑7901 gastric cancer cells. Oncology Letters. 10.3892/ol.2018.9045
