| Field | Specification |
|---|---|
| Target | |
| Alternative names | Extracellular calcium-sensing receptor; CaR |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human CASR recombinant protein (Position: Q926-S1078). Human CASR shares 80.5% and 78.6% amino acid (aa) sequence identity with mouse and rat CASR, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 11E9 is a mouse monoclonal antibody that recognizes extracellular calcium-sensing receptor (gene CASR) in human samples and is validated for Western blot. The predicted molecular weight is 120.7 kDa, and the supplier reports an observed band at about 130 kDa.
The calcium-sensing receptor (CaSR) is a class C G protein-coupled receptor that detects extracellular Ca2+. In the parathyroid gland, its activation suppresses parathyroid hormone secretion, and in the kidney it regulates calcium reabsorption, making it central to systemic calcium homeostasis. Inactivating and activating CASR mutations cause hypercalcemic and hypocalcemic disorders.
| Target | Extracellular calcium-sensing receptor (gene CASR; UniProt P41180, human) |
|---|---|
| Clone | 11E9 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 120.7 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human 22RV1 cells, human HepG2 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human CASR fragment (Gln926–Ser1078), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 80.5% and 78.6%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places extracellular calcium-sensing receptor in the cell membrane. Tissue expression noted by UniProt (human): Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
