| Field | Specification |
|---|---|
| Target | |
| Alternative names | Catechol O-methyltransferase;2.1.1.6;COMT; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human COMT. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes catechol O-methyltransferase (gene COMT) in human, mouse and rat samples and is validated for Western blot and IHC.
Catechol O-methyltransferase (COMT) transfers a methyl group from S-adenosylmethionine to catechols, inactivating dopamine, norepinephrine, epinephrine and catechol estrogens. It also metabolizes drugs such as L-DOPA, and COMT is widely studied in neuropharmacology, prefrontal dopamine signaling and Parkinson disease therapy.
| Target | Catechol O-methyltransferase (gene COMT; UniProt P21964, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
Antigen retrieval (datasheet): required for paraffin sections – boil in 10 mM citrate buffer (pH 6.0) for 20 minutes.
Immunogen
Synthetic peptide from the N-terminal region of human COMT.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Brain, liver, placenta, lymphocytes and erythrocytes.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
