| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cathepsin B;3.4.22.1;APP secretase;APPS;Cathepsin B1;Cathepsin B light chain;Cathepsin B heavy chain;CTSB;CPSB; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Cathepsin B |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CTSB antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AAFO-3; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody catalog # M01456. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CTSB (Cathepsin B).
- Antibody format: Monoclonal; clone AAFO-3; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CTSB (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Reported cellular localization context: Lysosome. Melanosome. Secreted, extracellular space . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue expression notes (as provided): Found on monocytes, neutrophils and eosinophil platelets.
Research relevance and current trends
- Research context keywords from the source record include: Angiogenesis,Cancer,Cell Adhesion,Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Membrane Proteins,Neuroscience,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate CTSB antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CTSB expression by Western blot in cell or tissue lysates, Detect CTSB in FFPE tissue sections by immunohistochemistry, Localize CTSB by immunofluorescence/immunocytochemistry in cultured cells, Enrich CTSB by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 38 kDa; calculated MW: 37822 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 38 kDa
- Cellular localization (provided): Lysosome. Melanosome. Secreted, extracellular space . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Tissue details (provided): Found on monocytes, neutrophils and eosinophil platelets.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
