| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cathepsin G;CG;3.4.21.20;CTSG; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Cathepsin G recombinant protein (Position: A76-L255). Human Cathepsin G shares 66% amino acid (aa) sequence identity with mouse Cathepsin G. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cathepsin G (gene CTSG) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 28.8 kDa, and the supplier reports an observed band at about 29 kDa.
Cathepsin G (CTSG) is a serine protease stored in the azurophil granules of neutrophils, with both trypsin-like and chymotrypsin-like activity. Released at sites of inflammation, it kills bacteria, remodels tissue, cleaves protease-activated receptors on platelets and other cells, and processes cytokines such as IL-36γ. It is studied in neutrophil biology, inflammation and host defense.
| Target | Cathepsin G (gene CTSG; UniProt P08311, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 28.8 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human THP-1 cells, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Cathepsin G fragment (Ala76–Leu255), expressed in E. coli. Sequence identity with the mouse ortholog: 66%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt describes cathepsin G as secreted and also located in the cell membrane, cytoplasmic granule and cytoplasm. Tissue expression noted by UniProt (human): Expressed in neutrophils (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
