| Field | Specification |
|---|---|
| Target | |
| Alternative names | Procathepsin L; Cathepsin L1; Major excreted protein; MEP; CTSL; CTSL1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Cathepsin L/MEP/CTSL, which shares 61.1% amino acid (aa) sequence identity with rat CTSL. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes procathepsin L (gene CTSL) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 37.6 kDa, and the supplier reports an observed band at about 37 kDa.
Cathepsin L (CTSL) is a lysosomal cysteine protease that drives general protein turnover. It also has specialized roles: processing antigens and the invariant chain for MHC class II presentation in the thymus, generating neuropeptides from their precursors, and degrading elastin and other matrix components. Cathepsin L also helps some viruses enter cells, so it is studied broadly.
| Target | Procathepsin L (gene CTSL; UniProt P07711, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 37.6 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|
Samples with a confirmed band (WB): human HepG2 cells, human hcct tissue, human hccp tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human cathepsin L/MEP/CTSL. Sequence identity with the rat ortholog: 61.1%.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes procathepsin L as secreted and also located in the lysosome and apical cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
