| Field | Specification |
|---|---|
| Target | |
| Alternative names | Procathepsin L; Cathepsin L1; Major excreted protein; MEP; p39 cysteine proteinase; Ctsl; Ctsl1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse Cathepsin L/MEP/Ctsl, which shares 70% and 95% amino acid (aa) sequence identity with human CTSL, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes procathepsin L (gene Ctsl) in mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 37.5 kDa, and the supplier reports an observed band at about 20–42 kDa.
Cathepsin L (CTSL) is a lysosomal cysteine protease that drives general protein turnover. It also has specialized roles: processing antigens and the invariant chain for MHC class II presentation in the thymus, generating neuropeptides from their precursors, and degrading elastin and other matrix components. Cathepsin L also helps some viruses enter cells, so it is studied broadly.
| Target | Procathepsin L (gene Ctsl; UniProt P06797, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 37.5 kDa |
| Observed MW | 20–42 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
Samples with a confirmed band (WB): rat liver tissue, rat kidney tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HEPA1–6 cells.
Immunogen
Synthetic peptide from the middle region of mouse cathepsin L/MEP/Ctsl. Sequence identity with the human ortholog: 70%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes procathepsin L as secreted and also located in the lysosome and apical cell membrane. Tissue expression noted by UniProt (mouse): Expressed in thymus, kidney and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Zhantao et al. (2025) Antimicrobial peptide CRAMP/LL-37 mediates ferroptosis resistance in cardiomyocytes by inhibiting cathepsin L. Basic Research in Cardiology. 10.1007/s00395-025-01122-z
