| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caveolin-1; CAV1; CAV |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat Caveolin-1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 12C7 is a mouse monoclonal antibody that recognizes caveolin-1 (gene CAV1) in human samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 20.5 kDa, and the supplier reports an observed band at about 22 kDa.
Caveolin-1 (CAV1) is the main structural protein of caveolae, small flask-shaped invaginations of the plasma membrane. It forms oligomers with caveolin-2, recruits cavin proteins, and acts as a scaffold for signaling molecules such as endothelial nitric oxide synthase and G proteins. It is widely studied in endothelial biology, lipid regulation, mechanosensing and cancer.
| Target | Caveolin-1 (gene CAV1; UniProt Q03135, human) |
|---|---|
| Clone | 12C7 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 20.5 kDa |
| Observed MW | 22 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, human placenta tissue, human U-87 cells, human PC-3 cells, human U2OS cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue, human lung cancer tissue, human melanoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (IHC): human placenta tissue.
Samples with confirmed staining (ICC/IF): human colorectal cancer tissue, human lung cancer tissue.
Immunogen
Recombinant human Caveolin-1 fragment (Gly4–Ile178), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95% and 94%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places caveolin-1 in the Golgi apparatus membrane, cell membrane, membrane and membrane raft. Tissue expression noted by UniProt (human): Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gao Jiale et al. (2025) Browning effect of LncRNA00602 in Ad36-induced differentiated adipocytes. Medicine. 10.1097/MD.0000000000043816
Wang Ning-Ning et al. (2013) Mechanistic Analysis of Taxol-induced Multidrug Resistance in an Ovarian Cancer Cell Line. Asian Pacific Journal of Cancer Prevention. 10.7314/APJCP.2013.14.9.4983
