| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caveolin-1;CAV1;CAV; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Caveolin-1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GE-3 is a rabbit monoclonal antibody that recognizes caveolin-1 (gene CAV1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 20.5 kDa, and the supplier reports an observed band at about 20 kDa.
Caveolin-1 (CAV1) is the main structural protein of caveolae, small flask-shaped invaginations of the plasma membrane. It forms oligomers with caveolin-2, recruits cavin proteins, and acts as a scaffold for signaling molecules such as endothelial nitric oxide synthase and G proteins. It is widely studied in endothelial biology, lipid regulation, mechanosensing and cancer.
| Target | Caveolin-1 (gene CAV1; UniProt Q03135, human) |
|---|---|
| Clone | GE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.5 kDa |
| Observed MW | 20 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with confirmed staining (IHC): mouse lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): A431 cells.
Immunogen
Synthetic peptide based on the sequence of human caveolin-1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places caveolin-1 in the Golgi apparatus membrane, cell membrane, membrane and membrane raft. Tissue expression noted by UniProt (human): Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
