| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mitogen-activated protein kinase 7;MAP kinase 7;MAPK 7;2.7.11.24;Big MAP kinase 1;BMK-1;Extracellular signal-regulated kinase 5;ERK-5;MAPK7;BMK1, ERK5, PRKM7; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human CBR1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CBR1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 26C20; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-CBR1 Rabbit Monoclonal Antibody catalog # M02825-2. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CBR1 (Mitogen-activated protein kinase 7).
- Antibody format: Monoclonal; clone 26C20; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CBR1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction. . Reported cellular localization context: Cytoplasm. Nucleus. Nucleus, PML body. Translocates to the nucleus upon activation. Tissue expression notes (as provided): Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. .
Research relevance and current trends
- Research context keywords from the source record include: Intracellular Signaling,Kinases,MAPK Pathway,Neural Signal Transduction,Neurology Process,Neuroscience,Neurotransmission,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate CBR1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CBR1 expression by Western blot in cell or tissue lysates, Enrich CBR1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 30 kDa; calculated MW: 88386 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 30 kDa
- Cellular localization (provided): Cytoplasm. Nucleus. Nucleus, PML body. Translocates to the nucleus upon activation.
- Tissue details (provided): Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
