| Field | Specification |
|---|---|
| Target | |
| Alternative names | Carbonyl reductase [NADPH] 1; 15-hydroxyprostaglandin dehydrogenase [NADP(+)]; EC 1.1.1.196, EC 1.1.1.197; 20-beta-hydroxysteroid dehydrogenase; Alcohol dehydrogenase [NAD(P)+] CBR1; NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; PG-9-KR; Prostaglandin-E(2) 9-reductase; Short chain dehydrogenase/reductase family 21C member 1; CBR1; CBR; CRN; SDR21C1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CBR1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26C20 is a rabbit monoclonal antibody that recognizes carbonyl reductase (gene CBR1) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 30.4 kDa, and the supplier reports an observed band at about 30 kDa.
Carbonyl reductase 1 (CBR1) is an NADPH-dependent cytosolic enzyme of the short-chain dehydrogenase/reductase family. It reduces a wide range of carbonyl compounds, including prostaglandins, quinones and xenobiotics, and converts the anthracyclines doxorubicin and daunorubicin into cardiotoxic alcohol metabolites. It is studied in drug metabolism and anthracycline cardiotoxicity.
| Target | Carbonyl reductase (gene CBR1; UniProt P16152, human) |
|---|---|
| Clone | 26C20 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.4 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CBR1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places carbonyl reductase in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in kidney (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
