| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; CBS |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CBS recombinant protein (Position: K102-E342). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 5B5D7 is a mouse monoclonal antibody that recognizes cystathionine beta-synthase (gene CBS) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 60.6 kDa, and the supplier reports an observed band at about 61 kDa.
Cystathionine β-synthase (CBS) is a pyridoxal phosphate-dependent enzyme that catalyzes the first step of the transsulfuration pathway, joining homocysteine and serine to form cystathionine. This removes homocysteine and supplies cysteine, and CBS also produces hydrogen sulfide. Inherited CBS deficiency is the most common cause of classical homocystinuria.
| Target | Cystathionine beta-synthase (gene CBS; UniProt P35520, human) |
|---|---|
| Clone | 5B5D7 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 60.6 kDa |
| Observed MW | 61 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human K562 cells, rat liver tissue, rat pancreas tissue, mouse liver tissue, mouse pancreas tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human CBS fragment (Lys102–Glu342), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cystathionine beta-synthase in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
