| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; CBS |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CBS |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20C55 is a rabbit monoclonal antibody that recognizes cystathionine beta-synthase (gene CBS) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 60.6 kDa, and the supplier reports an observed band at about 61 kDa.
Cystathionine β-synthase (CBS) is a pyridoxal phosphate-dependent enzyme that catalyzes the first step of the transsulfuration pathway, joining homocysteine and serine to form cystathionine. This removes homocysteine and supplies cysteine, and CBS also produces hydrogen sulfide. Inherited CBS deficiency is the most common cause of classical homocystinuria.
| Target | Cystathionine beta-synthase (gene CBS; UniProt P35520, human) |
|---|---|
| Clone | 20C55 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 60.6 kDa |
| Observed MW | 61 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CBS.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cystathionine beta-synthase in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
