| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 SUMO-protein ligase CBX4; Chromobox protein homolog 4; Polycomb 2 homolog; Pc2; hPc2; CBX4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CBX4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23C16 is a rabbit monoclonal antibody that recognizes E3 SUMO-protein ligase CBX4 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 61.4 kDa, and the supplier reports an observed band at about 50 kDa.
CBX4 (Pc2) is a Polycomb group protein of the PRC1 complex that recognizes methylated histone H3 through its chromodomain. It is also an E3 SUMO ligase that sumoylates proteins such as CtBP and proteins involved in DNA repair. CBX4 is studied in gene silencing, stem cell maintenance and the DNA damage response.
| Target | E3 SUMO-protein ligase CBX4 (gene CBX4; UniProt O00257, human) |
|---|---|
| Clone | 23C16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 61.4 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human 293T cells, human Jurkat cells, human HepG2 cells, human K562 cells, rat C6 cells, mouse kidney tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CBX4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places E3 SUMO-protein ligase CBX4 in the cytoplasm, nucleus and nucleus speckle. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
