| Field | Specification |
|---|---|
| Target | |
| Alternative names | BRCA1-A complex subunit Abraxas 1; Coiled-coil domain-containing protein 98; Protein FAM175A; ABRAXAS1; ABRA1; CCDC98; FAM175A |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CCDC98 |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28A03 is a rabbit monoclonal antibody that recognizes BRCA1-A complex subunit Abraxas 1 (gene ABRAXAS1) in human samples and is validated for Western blot, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 46.7 kDa, and the supplier reports an observed band at about 50 kDa.
Abraxas 1 (ABRAXAS1, CCDC98) is the scaffold subunit of the BRCA1-A complex. It recruits BRCA1 to sites of DNA double-strand breaks marked by K63-linked ubiquitin chains on histones, supporting the DNA damage checkpoint and repair, and it is studied in genome stability and breast cancer.
| Target | BRCA1-A complex subunit Abraxas 1 (gene ABRAXAS1; UniProt Q6UWZ7, human) |
|---|---|
| Clone | 28A03 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.7 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CCDC98.
Reactivity Notes
The supplier lists reactivity with human. UniProt places BRCA1-A complex subunit Abraxas 1 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
